Comparison of conventional immunochromatographic assay with new automated-Treponema pallidum hemagglutination assay for screening of syphilis in blood donors
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چکیده
منابع مشابه
Screening tests for syphilis. A comparison of the Treponema pallidum haemagglutination assay with two automated serological tests.
Two Treponema pallidum haemagglutination assay (TPHA) tests (Wellcome and Fuzizoki) were compared with the automated reagin test and the automated Reiter protein complement-fixation test in a routine screening system. The Wellcome TPHA was shown to lack sensitivity, and it is recommended that it should be used at a lower screening dilution. The Fuzizoki TPHA recorded a significant number of fal...
متن کاملTreponema pallidum surface immunofluorescence assay for serologic diagnosis of syphilis.
A surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and Treponema pallidum immobilization (TPI) assays for detecting serum antibodies to T. pallidum in patients with syphilis, in disease controls, and in healthy subjects. SIFA and WB were 99% sensi...
متن کاملQuantitative microhaemagglutination assay for Treponema pallidum antibodies in experimental syphilis.
The quantitative microhaemagglutination assay for Treponema pallidum antibodies (MHA-TP) was studies in 52 untreated and treated rabbits with experimental syphilis. Rabbits with incubating experimental syphilis were cured or inadequately treated with penicillin G and some cured rabbits were later reinfected. MHA-TP conversion occurred within 45 days in untreated rabbits. Titres reached peak lev...
متن کاملAutomated, quantitative microhemagglutination assay for Treponema pallidum antibodies.
An automated, quantitative microhemagglutination assay for antibodies to Treponema pallidum was developed by using T. pallidum-sensitized erythrocytes and an automatic serial-dilution instrument. Reactivity was found in sera from 54 rabbits and 6 chimpanzees infected with T. pallidum. Reactivity was also found in sera from animals infected with T. pertenue, T. carateum, and T. cuniculi. No reac...
متن کاملPerformance evaluation of the Elecsys syphilis assay for the detection of total antibodies to Treponema pallidum.
Syphilis is a health problem of increasing incidence in recent years that may have severe complications if not diagnosed and treated at an early stage. There are many diagnostic tests available for syphilis, but there is no gold standard, and diagnosis therefore usually relies upon a combination of tests. In this multicenter study, we evaluated the treponemal Elecsys syphilis assay for use in t...
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ژورنال
عنوان ژورنال: Global Journal of Transfusion Medicine
سال: 2019
ISSN: 2468-8398
DOI: 10.4103/gjtm.gjtm_55_19